WORKSHEET: EXERCISE 2 NAME: SECTION: DATE: QUESTIONS (1 pt each) You want to foc
ID: 217421 • Letter: W
Question
WORKSHEET: EXERCISE 2 NAME: SECTION: DATE: QUESTIONS (1 pt each) You want to focus on a prepared slide. Describe the steps necessary to ultimately focus a microscope with the high dry objective after placing the slide on the stage. 1. 2. When moving from 4X to 10X your slide stays in focus. What term is used to describe this? What are the rules about using the coarse and fine focus knobs? 3. How is total magnification calculated? Provide examples tal mod itionmog nification o alar lens x nognifica-tion ?objective ens Exercise 2 Introduction to the MicroscopeExplanation / Answer
1) Steps in Using the Microscope:
1. Wipe the lenses with lens paper
2. Plug in the microscope and switch on the light
3. Open the diaphragm around midway and place the condenser so
it is just under the stage
4. Keep open the mechanical stage lever and put a slide in the
slide holder
5. Utilize the mechanical stage knobs to centre the slide so the
specimen is placed right over the condenser lens
6. Always start focusing on employing the scanning (4X) or low power
(10X) objective and use the coarse adjustment knob to bring the
stage as close to the objective lens as possible
7. While watching through the ocular lens, gradually back away using the coarse adjustment knobs till the specimen comes into focus.
using the fine adjustment knobs Fine focus the image
8. Switch to the high dry (40X) objective and fine focus utilizing the fine
adjustment knobs this time you do not need to use the coarse adjustment knobs because your microscope is parfocal.
2) Parfocal means the image will remain in focus when you switch from low power to high power.
when using a microscope you should Always start with the coarse adjustment knob and the low power objective lens. Later proceed with the medium objective lens and fine adjustment knob. Then use the high objective lens and fine adjustment knob.the coarse adjustment knob should not be used for medium and high power objective lenses.
3) Total magnification of an object seen through the eyepieces (ocular lens) can be calculated by multiplying the ocular lens magnification times the magnification of the objective lens being used (either: 4X, 10X, 20X, 40X, 100X, etc.). X is placed after receiving the total magnification number.
example:
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.