Recombinant DNA technology Q2. Restriction enzymes are extensively used in molec

Question

Recombinant DNA technology

Q2. Restriction enzymes are extensively used in molecular biology. The restriction enzymes Sal Iand Xho I cut their recognition sequences as shown below, where the * indicates where it is cut

CUTSITE (Enzyme Sal I)

5’----G*T C G A C----- 3’

3’----C A G C T*G-----5’

CUTSITE (Enzyme Xho I)

5’--- C*T C G A G ---3’

3’---G A G C T*C---5’

2a-2pt) Indicate the 5’ and 3’ ends of the cut molecules below [Both strands and both ends]

Enzyme Sal 1

----G                           T C G A C----

----C A G C T                            G----

Enzyme Xho I

----C                             T C G A G----

----G A G C T                             C----

Explanation / Answer

Enzyme Sal 1
5'-G-3' 5'-TCGAC-3'
3'-CAGCT-5' 3'-G-5'

Enzyme Xho I
5'-C-3' 5'-TCGAG-3'
3'-GAGCT-5' 3'-C-5'

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