50. The fluorescence reading in the figure from the previous question is being g

Question

50. The fluorescence reading in the figure from the previous question is being generated by:

A. The binding of SYBR Green to mRNA

B. The binding of SYBR Green to single-stranded DNA

C. The binding of SYBR Green to double-stranded DNA

D. The binding of SYBR Green to double-stranded RNA

E. The binding of SYPRO Ruby to mRNA

51. microRNA-27 binds the 3'-UTR of the mRNA for the receptor IGF-I. microRNA-27 also has a high degree of complementarity with a segment of the long non-coding RNA, IncRNA133.

Based on this information, you expect that an increase in microRNA-27 would cause the level of IGF-I protein toand an increase in IncRNA-133 would cause the level of IGF-I protein to

A. increase, increase

B. decrease, decrease

C. increase, decrease

D. decrease, increase

E. decrease, remain unchanged

52. Which of the following is an allosteric effector involved in the regulation of the Lac operon.

A. the Lac repressor.

B. allolactose.

C. cAMP receptor protein.

D. galactose.

E. ß-galactosidase.

53. A single nucleotide insertion mutation holds the potential to alter how many amino acids in the resultant protein:

A. One amino acid

B. Two amino acids

C. Three amino acids

D. More than three amino acids

E. No amino acids, the mutation would be silent

54. Differential RNA processing may result in:

A. a shift in the ratio of mRNA produced from two adjacent genes.

B. inversion of certain exons in the mature mRNA.

C. the crossover of exons between two different pre-mRNA transcripts

D. the production of the same protein from two different genes.

E. the production of two proteins with different activities from a single gene.

55. The bacterial Shine—Dalgarno mRNA sequence is specifically recognized by:

A. The 18S rNA of the 40S subunit

B. protein S5 of the 30S subunit.

C. N-formylmethionyl-tRNAfMet

D. the 23S rRNA of the 50S subunit.

E. the 16S rRNA of the 30S subunit.

56. Which of the following is true of the electrophoretic mobility shift assay but NOT the DNA footprinting assay:

A. the technique can be used to identify binding of a protein to a specific segment of DNA

B. the technique involves the resolution of samples by nondenaturing gel electrophoresis

C. the technique involves the amplification of radiolabeled DNA by PCR

D. the technique can identify the precise location of protein binding to a sequence of DNA

E. the technique involves the cleavage of protein-bound DNA using DNase I or free radicals

Explanation / Answer

50. To answer this question, we need to have question 49

51. An increase in microRNA-27 would cause the level of IGF-I protein to decrease and an increase in IncRNA-133 would cause the level of IGF-I protein to remain unchanged.

So the answer would be E (decrease, remain unchanged)

52. Allolactose is an allosteric effector which binds to the regulatory subunit of lac repressor and inhibits its binding to operator region.

So the answer is B (allolactose)

53. A single nucleotide insertion mutation can result in a change in the reading frame (frameshift mutation) and has the potential to alter multiple amino acids of the protein.

So, the answer is More than three amino acids (D)

54. Differential RNA processing also called as alternative splicing or differential splicing result in the production of two proteins with different activities from a single gene.

So, the answer is E

55. the 16S rRNA of the 30S subunit (Answer E)

56. EMSA is used to identify binding of a protein to a specific segment of DNA (Answer A).

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