Western blot, Southern blot, Sedimentation Gradient, DNA footprinting, PCR, or N
ID: 216790 • Letter: W
Question
Western blot, Southern blot, Sedimentation Gradient, DNA footprinting, PCR, or Next generation sequencing.
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Passage I cDNA and gene expression Questions 8-10 cDNA for twelve different liver specific genes was spotted onto a membrane as shown in the bottom left corner. cDNA for the actin, the alpha subunit of tubulin (used to make microtubules), and the beta subunit of tubulin genes were spotted as shown. The DNA from the plasmid pB (used as a vector to carry the cDNA) was spotted on the membrane as shown. DNA for the methionine tRNA gene was spotted on the membrane as shown. The DNA was denatured (single stranded) and remained tightly bound to the membrane. Three replicate spotted membranes were produced A rat was fed 32P-labeled UTP for several days (the rat now was undesirable for eating.... The rat was sacrificed and the total RNA from the rat liver tissue, the rat kidney tissue, and the rat brain tissue were separately isolated. The radioactive RNA from each tissue type was used as a probe to hybridize to the DNA targets on the membrane. The autoradiograms are shown above for the liver, kidney, and brain probe conditions. Kidney ANA Liver Liver cONAs Braln RNA 10 12 The technique used in this experiment is best named by which one of the following options?Explanation / Answer
In this question take each option one by one
Western blot is generally used for protein detection with the help of antibodies. Here radioactive DNA is used that is used in southern blotting. Southern blotting is correct answer.
In sedimentation gradient, sucrose gradient is used for the isolation of various components of the cell. That description is not given.
DNA foot printing is used for the DNA protein interaction. Here nucleic acid used.
PCR used for amplification and that type of lablelling through PCR is not given.
Next generation sequencing is used for the genome sequencing and that is not mentioned in procedure.
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