Pretend that you setup an experiment to study the gene expression of HSV-1. You
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Pretend that you setup an experiment to study the gene expression of HSV-1. You infect 4 dishes of a permissive cell line with this virus. You add 3 different inhibitors during the infection. Drug A prevents all host cell translation. Drug B inhibits viral DNA replication. Drug C prevents the assembly of viral capsids. You then measure how much viral mRNA is synthesized from 3 different viral genes. See the table below of the results: 3. | Inhibitor Viral gene #1 | Viral gene #2 | Viral gene #3 None (control) "+" signifies the relative amount of viral mRNA transcribed at each time point "means that no viral mRNA was detected in that condition (a) At what time point do you think each viral gene is synthesized during the infection cycle of HSV? (Note: you do not need to give an actual time (such as hours), just the name of the class of gene.) [6 pts.] Viral gene #1 : Viral gene #2: Viral gene #3: (b) Why does drug A not prevent transcription from viral gene #1? Explain. [4 pts.]Explanation / Answer
A.
Viral gene second.
In this we can say, that gene 2 have the steps of gene synthesis and after the step of gene 2 all RNA will be start synthesizing. As the table indicates that gene 1 step have all synthesis of RNA. Gene third is considered as last step.
Follow the amount of RNA because RNA is synthesized after DNA. so gene one is indicating that all RNA is synthesized means gene one is second condition.
b. Drug A is associated with the inhibition of translation process. Translation is the process of synthesis of protein from RNA.
The gene is able to produce RNA by the process of transcription.
This RNA molecule convert Amino acid sequence to a long polypeptide chain known as protein by the process of translation.
So translation is the next procedure of transcription.
So gene A inhibits translation process not transcription process.
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