When scientists tried to decipher the genetic code, they synthesized, let\'s say
ID: 215525 • Letter: W
Question
When scientists tried to decipher the genetic code, they synthesized, let's say, a poli-U mRNA. But how did they know that, in order for the ribosomes to start protein synthesis, that mRNA needed an AUG sequence first?
Also, in order to visualize the obtained protein, the scientists acidified the solution, precipitating the protein. But what makes a protein precipitate in an acidic solution? I mean, if the protein has (for example) only non-polar amino acids, why does it precipitate?
Thanks in advance!
Explanation / Answer
During translation initiation, first tRNA which have methionine or UAC will attach with the small subunit of ribosome. Next this complex can identify the 5' capped region or mRNA and bind at the 5' region. After this the complex starts to scan the entire mRNA sequence and will bind to the region where it will find codon AUG which is also known as start codon.
Acids like TCA or picric acid can precipitate proteins, This precipitation is carried through an association beteween anionic group and cationic group. Proteins have positive charge whereras these acids have large negative charge which is ued to neutralize the proteins and precipitate to form a insoluble complex of salt.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.