I am supposed to make different ligations. These are the stocks I am given: Cut

Question

I am supposed to make different ligations.
These are the stocks I am given:

Cut pET41a(+) DNA: 25 ng/ul
egfp insert DNA: 7 ng/ul
Uncut pET-41a(+)/EGFP recombinant plasmid DNA: 25 ng/ul

This is the ligation I am supposed to make:
Ligation #1: 1:1 molar ratio pET-41a(+) vector: egfp insert
50 ng NcoI/NotI cut pET-41a(+) DNA _______ ul
7 ng egfp insert DNA _____?l
sterile dH2O _____?l
10x ligase buffer _____ ul (final concentration will be 1X)
DNA ligase: 1 ul

The final volume for the ligation is 20ul.
I am trying to find out how ul of the stock I am supposed to add for the cut and I am having trouble. I am using the formula C1V1=C2V2. I know the right answer is supposed to be 2 ul but when I solve I keep getting 40 ul. I am using C1=25ng/ul, C2=50ng, V1 is what Im trying to find out, and V2=20ul. When I solve it though I keep getting 40ul. What am I doing wrong?

Explanation / Answer

I Believe C1 = 25 ng and C2 = 50 ng and V1 = 1 uL and you are trying to find V2?

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