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(2 pts) The DNA of bacteriophage genomes are either single- or double-stranded.

ID: 215427 • Letter: #

Question

(2 pts) The DNA of bacteriophage genomes are either single- or double-stranded. The base composition of eight acteriophage DNAs are shown in the table below. BASE COMPOSITION Phage 19.8% Pf3 P4 C2 Ike PZA S13 fd 21.3% 24.8% 18.2% 25.2% 31.8% 25.1% 30.2% 23.9% 24.6% 30.6% 24.1% 24.8% 18.2% 2 1.6% 19.8% 23.3% 25.2% 3 1.8% 34.3% 18.9% 19.8% 21.1% 20.2% 19.4% 30.2% 3 1.7% 20.75% 19.4% 34.5% 30.6% Which of these bacteriophage most likely contain single-stranded DNA within the virus? HOW DID YOU REACH THIS CONCLUSION???? EXPLAIN. What is the name of the rule being applied to address this question (it is based on the name of the scientist that first described the base composition of DNA).

Explanation / Answer

Chargaff's Rule says, if the DNA is double-stranded A will pair with T, G will pair with C, hence [A] = [T] and [G] = [C]

We can see from above data that Pf3 shows maximum deviation from the rule and hence is likely to have single-stranded DNA