Question 7 (5 points) The GAL4-UAS system is a powerful genetic tool allowing th

Question

Question 7 (5 points) The GAL4-UAS system is a powerful genetic tool allowing the ectopic expression of any given sequence, be it protein-coding or a noncoding RNA (e.g., RNAi). Which of the following statements best describes how this system works? A fly carrying a transgene with GAL4 fused to a promoter of interest is crossed to a fly carrying a transgene with a GAL4 binding site in its promoter. A fly carrying a transgene with GAL4 fused to a promoter of interest is crossed to a fly carrying a transgene with a heat-shock promoter. A fly carrying a transgene with a GAL4 binding site in its promoter is exposed to heat shock. A promoter linked to a GAL4-GFP fusion is inserted into a fly. Save

Explanation / Answer

The GAL-UAS system is a powerful genetic tool which allows one to study the ectopic expression of
our gene of interest.
The GAL4 gene is placed under the control of a promotor and UAS gene regulates the target gene expression
. The system is often fused with GFP, by which the expression of target genes can be measured.
Option d is the answer.

Influence of one cell over another neighboring cell's development is called as induction.
Cells which show influence are called as organizers.
Option c is the answer.

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