Question 5 (1 point) Brain-specific enhancer Limb-specific enhancer lacZ mRNA ex

Question

Question 5 (1 point) Brain-specific enhancer Limb-specific enhancer lacZ mRNA expressed You have made a reporter construct as shown in the figure below. You look at embryos expressing this construct and stained for lacZ in a blastocyst and a developmental stage when all organ primordia are present. Where do you predict you will see expression? a) ubiquitous expression in blastocyst; only in the brain and limb in later embryo b) no expression in blastocyst; in the brain and limb in later embryo O c) in a few pockets of cells that are the precursors to the limb in the blastocyst; in the brain and limb later O d) no expression in blastocyst; low level expression everywhere later, but highest in brain and limb Save

Explanation / Answer

question 5. Answer (d)

since the construct has brain and limb specific ehancers it will be expressed everywhere but the expression will be enhanced in brain and limbs. In the blastocyct which is the stage when implantation occurs, no expression will be seen.

questin 6. Answer (d)

The nematode uses RNA pol II for transcription. But for the first 6 cleavages zygotic genome is not required, maternal products – proteins and mRNAs contribute to the division.

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