3. One round of PCR is carried in three sequential steps: a heating step, a cool

Question

3. One round of PCR is carried in three sequential steps: a heating step, a cooling step, and a step during which new DNA is synthesized. What is the purpose of the cooling step? Cooling the reaction ensures the integrity of the covalent bonds holding the nucleotides together in the DNA strand. a. b. Cooling the reaction gives the DNA polymerase an opportunity to recover from the previous PCR cycle such that it will be able copy DNA in the subsequent step of the reaction. c. Transcription takes place during the cooling step d. Cooling the reaction brings the temperature down to a level that is compatible with the binding of the primers to the template DNA being amplified.

Explanation / Answer

3. The answer is d.Cooling the reaction bring the temperature down at a level that is compatible with the binding of the primers to the template DNA being modified.

The cooling temperature is depends on the sequence and length of the primers. It varies from primers to primers.

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