6. The following questions are in regard to the genetic code. (a) What does \"de

Question

6. The following questions are in regard to the genetic code. (a) What does "degeneracy" mean in the context of the genetic code? (b) You have discovered a protein that performs an interesting function. After purifying the protein and performing "Edman degradation." you have determined that the following sequence of amino acids is present within the sequence of your purified protein: MVSKGEELFT Where is this sequen ctwithn your protein? (check one) N-terminus C-terminus Cannot be determined Using the amino acid sequence provided in (b), you set out to design a set of degenerate primers in order to amplify the cDNA sequence corresponding to your protein of interest from a cDNA library. In order to do so, you need to synthesize all the oligonucleotide primers that may possess complementary to the 5' and 3' ends of your targeted gene. Assuming you chose a length of 20 bases for your "forward" primer, how many possible degenerate sequences would you need to create in order to cover all possible sequences corresponding to your protein? (c) List the nucleotide sequence of one of your designed primers below. Be sure to label the 5' and 3' ends. (d)

Explanation / Answer

(b) Sequence will be N-terminal because it is derived from Edman degradation and protein derived from this method residue present at amino terminal is cleaved from N-terminal.

(c) 5 possible degenerate sequences .

(d) ATCGTT[GC]AAGT[AGC]ATC

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