1) A polylinker (also known as a multiple cloning site) is a: A) synthetic DNA f
ID: 214818 • Letter: 1
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1) A polylinker (also known as a multiple cloning site) is a: A) synthetic DNA fragment that contains multiple restriction enzyme sites B) synthetic DNA fragment inserted into a vector that allows replication of the vector in the host cell C) DNA sequence that allows for selection of cells that contain the vector D) molecule used to ligate DNA fragments together E) synthetic DNA fragment used to screen or visualize colonies that contain the DNA of interest 2) Generating a recombinant DNA vector in vitro with a fragment of chromosomal DNA requires A) DNA polymerase B) DNA ligase C) restriction enzymes D) B and C E) A, B and C 3) You are a graduate student studying the human UCLA gene. Your first task is to clone the UCLA gene into a plasmid vector and generate bacterial cells that carry the recombinant DNA vector with the UCLA gene. The plasmid vector you are using includes a resistance gene for the antibiotic Kanamycin. The vector also contains the lacZ gene region encoding -galactosidase with an internal Polvinker inserted within the genetic sequence, which allows you to do BLUE-WHITE screening as was discussed in lecture. You insert the UCLA gene into the polylinker to make recombinant DNA. After performing bacterial transformation with your recombinant vector you plate the bacterial cells on a medium containing Kanamycin and X-gal (an analog of lactose). The blue bacterial colonies that you observe represent while the white bacterial colonies that you observe represent T'empty vector"-plasmid vector with no DNA insert] A) bacterial cells with a recombinant vector; bacterial cells with an empty vector B) bacterial cells with an empty vector; bacterial cells with a recombinant vector C) bacterial cells with no vector; bacterial cells with a recombinant vector D) bacterial cells with no vector; bacterial cells with an empty vector E) bacterial cells with a recombinant vector; bacterial cells with no vectorExplanation / Answer
1. Option [A] synthetic DNA fragment that contains various restriction enzyme sites.
Reason- Polylinkers are engineered plasmids which contain many restriction sites. It is a short segment of DNA.
2] Option [E] A, B and C
Reason-- Restriction enzymes or 'molecular scissors' cut the DNA molecule at certain sites.
DNA polymerase help in amplification of specific DNA sequences .
DNA ligase helps to mix vector DNA and gene of interest.
3]
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