Question 21 You have created a cell-free ( in vitro ) transcription system that

Question

Question 21

You have created a cell-free (in vitro) transcription system that contains a bacterial gene. It is able to initiate transcription but does so at random locations on the DNA. Which of the following proteins is most likely missing from the reaction?

Sigma factor

Rho factor

RNA polymerase

5' Cap proteins

DNA polymerase

1 points

Question 22

The genetic code is said to be degenerate because:

there are more codons than amino acids.

there are more amino acids than codons.

different organisms use different codons to encode the same amino acid.

there are more tRNAs than amino acids.

None of the above.

1 points

Question 23

The function of aminoacyl-tRNA synthetases is to:

transcribe tRNA genes.

match tRNA anticodons and mRNA codons at the ribosome.

attach appropriate amino acids to corresponding tRNAs.

form the peptide bond between amino acids at the ribosome.

synthesize and transport amino acids to the ribosomes.

Question 25

An mRNA has the stop codon 5' UAA 3'. What tRNA anticodon will bind to it?

5' ATT 3'

5' AUC 3'

5' ACU 3'

5' UUA 3'

None

Sigma factor

Rho factor

RNA polymerase

5' Cap proteins

DNA polymerase

Explanation / Answer

25. Ans- none

tRNA anticodon will bind to 3’ AUU 5’. If mRNA codes in 5’-3’ direction, then anticodon codes in 3’-5’ direction. In mRNA, A= U and G=C. Thymine is replaced by uracil here.

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