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Question 1 (2 points) Questions 1 through 4 refer to the following problem: In c

ID: 214639 • Letter: Q

Question

Question 1 (2 points) Questions 1 through 4 refer to the following problem: In corn, the following recessive genes have been identified on chromosome 3: yg yellow-green seedlings wx waxy endosperm bz bronze color From a three-point testcross between plants heterozygous for all three genes and plants homozygous recessive for all three genes, the following data were obtained: yellow-green - 6 yellow-green, waxy 720 wild type 56 bronze, waxy 13 bronze -681 yellow-green, bronze -25 waxy - -31 yellow-green, bronze, waxy - 42 Which gene is in the middle? yg bz wx

Explanation / Answer

Answer:

1). Middle gene = wx

2). Distance between genes yg and wx is 4.8 m.u.

3). Distance between genes wx and bz is 7.4 m.u.

Explanation:                                       

Hint: Always recombinant genotypes are smaller than the non-recombinant genotypes.

Hence, the parental (non-recombinant) genotype is yg bz+ wx / yg+ bz wx+

1).

If single crossover occurs between yg& bz+

Normal combination: yg bz+ / yg+ bz

After crossover: yg bz / yg+ bz+

yg bz progeny= 25+42=67

yg+ bz+ progeny = 31+56=87

Total this progeny = 154

Total progeny = 1574

The recombination frequency between yg & bz+ = (number of recombinants/Total progeny) 100

RF = (154/1574)100 = 9.78%

2).

If single crossover occurs between bz+ & wx..

Normal combination: bz+ wx / bz wx+

After crossover: bz+ wx+ / bz wx

bz+ wx+ = 6+56 = 62

bz wx progeny = 13+42=55

Total this progeny = 117

The recombination frequency between bz+ &wx = (number of recombinants/Total progeny) 100

RF = (117/1574)100 = 7.43% = 7.4%

3).

If single crossover occurs between yg & wx..

Normal combination: yg wx / yg+ wx+

After crossover: yg wx+ / yg+ wx

yg wx+ = 6+25 = 31

yg+ wx progeny = 13+31=44

Total this progeny = 75

The recombination frequency between yg &wx = (number of recombinants/Total progeny) 100

RF = (75/1574)100 = 4.77% = 4.8%

Recombination frequency (%) = Distance between the genes (mu)

yg-------4.8mu------wx---------7.4mu---------bz

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