In the process of DNA purification, what is the purpose of adding protease? To i

Question

In the process of DNA purification, what is the purpose of adding protease?

To interact with the negative charges of the phosphate groups in the DNA to neutralize its charge.

Remove chromatin proteins associated with the DNA

Pull water from the DNA molecule to allow the DNA to precipitate from the solution

Destroys the cell plasma membrane and nuclear envelope

To interact with the negative charges of the phosphate groups in the DNA to neutralize its charge.

Remove chromatin proteins associated with the DNA

Pull water from the DNA molecule to allow the DNA to precipitate from the solution

Destroys the cell plasma membrane and nuclear envelope

Explanation / Answer

In the process of DNA purification, what is the purpose of adding protease?

D) Destroys the cell plasma membrane and nuclear envelope

In order to get the DNA from the cells, we need to break the cells. We usually digest them with a proteinase (aka protease) that breaks down the structural proteins and allow the DNA out into the buffer, from there it can be retrieved.

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