5) Serine 795 (S795) of Rb is phosphorylated by CDK4, and this leads to Rb inact

Question

5) Serine 795 (S795) of Rb is phosphorylated by CDK4, and this leads to Rb inactivation. Suppose you want to study the effects of persistent phosphorylation of Rb on Serine 795. You cannot easily regulate the phosphorylation of Serine 795, so you instead need to generate a mutation in the Rb gene that makes Serine 795 always appear to be phosphorylated. The codon that encodes for S795 in Rb is TCA. ? Explain your answer. Answer in no more than 2 sentences. (4 points) b) How would you mutate the codon that encodes for S795 (TCA) in Rb to achieve your goal for a)? (1 point)

Explanation / Answer

a) We can use codon TGA (amber stop codon) that codes for phosphoserine instead of serine. This way S795 can be altered to its phosphorylated derivative.

b) The mutation must cause C-G transversion. For this, both ionizing radiations or alkylating agents can be used.

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