Answer True (A) or False () for the following statements Use the sppropriate col

Question

Answer True (A) or False () for the following statements Use the sppropriate columms of the 31. With 32. 33. A chromosome has only one centromere the Chi Square test, chance deviation from an expected outcome is diminished by larger sample size. In the Chi Square test, more deviation is expected with a higher degree of freedom. the 3 criteria used to identify chromosomes are size, banding pattems, and chromatid curvature. 35. In karyotype preparation, the arrested cells are treated with a hypertonie solution to swell the nuclei. 36. Banding patterns on chromosome are produced as a result of differential staining between AT-rich sequences and GC-rich regions 37. The loss of one chromosome to produce a 2n-1 complement is called disomy 38. Karyotyping can be used as a diagnostic tool to identify abnormal chromosomal rearrangements. 39. The number of bands produced by staining is directly related to the size of the chromosome. 40. Each member of a pair of homologous chromosomes has a unique banding pattern. The Ames test is used extensively during the development of industrial and pharmaceutical chemical compounds as a preliminary assay for identifying carcinogens. 41. 42. ALL substances testing positive on the Ames test are highly likely to produce cancers in humans 43. In the Ames test, why is the potential mutagen mixed with liver extract? A. To neutralize the mutagenic properties of the test chemical B. The liver enzymes can generate metabolic products from the test chemical that can be themselves be mutagenic. C. The liver contains DNA modifying enzymes that protect the DNA from damage. D. The liver extract provides a source of histidine for the tester strains. 44. After performing an Ames test, a researcher observed the presence of several colonies on the test plate and a few sporadic colonies on the control plate. The presence of colonies on the control plate is best explained by A. Induced mutation B. Environmental contamination C. Excess histidine in the minimal media D. Spontaneous mutation 45. The tester strains used in the Ames test are nutritional mutants known as B. protrophs C. autotrophs D. heterotrophs 46. When his bacteria are mutated back to the wild-type state they are A. auxotrophs B. heterotrophs C. revertants D. colony-forming units END OF EXA

Explanation / Answer

31) False

32) True

33) True

34) False Karyotyping is done based on number, type shape and banding pattern of the chromosomes

35) False. Cells are arrested in metaphase

36) true

37) false- Disomy is the condition when a chromosome is represented twice in a complement. Monosomy is the loss ofone chromosome.

38) true

39) False- bands dends on the ration of A-T and G-C rich regions

40) False- they show same pattern

41) True

42) True

43) D

44) A

45) A auxotroph is a mutant organism that requires a particular additional nutrient which the normal strain cannot produce.

46) C

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