) Consider the following agarose solutions: 0.75% 1.25% and 1.50%, which one wil
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Question
) Consider the following agarose solutions: 0.75% 1.25% and 1.50%, which one will create a gel with the largest pore size? a) 075% b) 1.25% c) 1.50% d) the pore sizes will be the same if the same type of agarose is used for each solution 9) With respect to the alpha and beta globin genes, an individual that is heterozygous for sickle cell anemia has a) 4 normal globin alleles b) 4 abnormal globin alleles c3 normal globin alleles and I abnormal globin allele d) 2 normal globin alleles and 2 abnormal globin alleles 10) In what way does glycerol affect the charge of the sample being loaded into the well of a gel for electrophoresis? a) It neutralizes positive and negative charges b) It creates more negative charges c) It creates more positive charges d) It has no effect on the charge of the sampleExplanation / Answer
8. If the concentration of agarose increases, the pore size will be reduced. The relation between the concentration of agarose and poresize is indirectly proportional, So, the solution with 0.75% creates the gel with larget pore size here.
so, the answer is a. 0.752 %
9. The answer is d. 2 nomral globin alleles and 2 abrnormal globin alleles.
10. The answer is d. It have no effect on the charge of the sample
The Glycerol gives the density to a sample so the sample will not be disturbed by the tank buffer. In general 5-10% of the glycerol will be useful in sample prepration.
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