While working in a lab, you determine the overall critical concentration for a p

Question

While working in a lab, you determine the overall critical concentration for a preparation

of purified tubulin. You then add purified centrosome components which attach to the minus ends

of tubulin. You then remeasure the overall critical concentration, and you find that it has.....

1.increased, because the minus end cannot disassemble and the overall critical

concentration is now equal to that the higher value for the plus end

2. increased, because the minus end cannot disassemble, hence the critical

concentration will increase.

3.decreased, because the minus end cannot disassemble and the overall critical

concentration is now equal to that the lower value for the plus end

4.not changed at all

5. decreased, because centrosome components decrease the critical concentration at

the plus end

I know 1 is not the right answer, only one can be the right answer? Is it 2,3,4,5 please explain. Thank You.

Explanation / Answer

5) Decreased, because centrosome components decrease the critical concentration at the plus ends.

This is because this is a GTP driven process which is linked to tubulin assembly. It allows assembly of the centrosome to the minus ends of the tubulin which overall is responsible for decreased critical concentration.

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