1. You have a circular piece of DNA that can be cleaved by the restriction nucle
ID: 213850 • Letter: 1
Question
1. You have a circular piece of DNA that can be cleaved by the restriction nucleases EcoRI, HindIlI, and Notl at the positions indicated on the "plasmid map" below. Considering this plasmid map, which of the following statements would be untrue? Notl EcoRI EcoRI Hindilll Figure Q10-6 a. Cleavage of the plasmid with Notl will produce a single linear DNA product. b. Cleavage of the plasmid with Notl and HindIlI will produce multiple DNA c. Cleavage of the plasmid with EcoRI and Notl will produce multiple DNA d. Cleavage of the plasmid with HindlII and EcoRI will produce linear DNA e. None of the above segments, one of which will not be a substrate for EcoRI segments, one of which is not a substrate for HindIII. fragments of unequal size.Explanation / Answer
a. Not I has a single site. So, Not I digestion would linearize the plasmid.
b. Not I has a single site and HindIII also has a single site. So, Not I + HindIII digestion would produce two bands. The smaller fragment of this digestion contains two sites for EcoRI digestion. Hence option B is incorrect.
c. The largest fragment produced by Not I + EcoRI digestion contains a single site for HindIII. Hence option C is incorrect.
d. HindIII + EcoRI digestion produces three bands of unequal sizes.
e. None of the above is incorrect.
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