3. We discussed how the Gal4 transcription factor regulates the enzymes fequiref

Question

3. We discussed how the Gal4 transcription factor regulates the enzymes fequirefralactose metabolism, which includes the downstream target Gal2. Complete the following table. Insert a "+" where GAL2 activity would be seen and a"" where no enzyme is produced. GAL2 activity no galactose high galactose Wt yeast cells gal4-yeast cells gal3- yeast cells, defective in the galactose sensor Gal3 What feature(s) would you expect to see in the GAL2 gene that would influence its expression? How is the expression of all the genes acting in galactose metabolism coordinated?

Explanation / Answer

GAL2 codes for membrane bound subunit of galactose permease and it helps in expression. The GAL2 genes in presence of galactose will show expression and activity which helps in regulation of the transcription.

The expression of all the genes acting in galactose metabolism coordinated either in presence or absence of the element. When Gal3 is present, it helps in better co-ordination in presence of galactose.

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