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.\'ll AT&T; 9:40 PM genetics-quiz-6 PDF 63 KB Close NAME: BIO 2450-GENETICS LAB

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Question

.'ll AT&T; 9:40 PM genetics-quiz-6 PDF 63 KB Close NAME: BIO 2450-GENETICS LAB QUIZ 6 1 DNA consists of two strands. a. Complimentary and parallel c. Identical and parallel d. Identical and antiparallel 2. A purine nitrogenous base always pairs with a pyrimidine nitrogenous base. Identify which of the following pairings is correct (one answer). a AG and CT b. AC and GT c. AT and GC 3. Why is DNA replication considered to be semi-conservative? (short answer- no more than 20 words) -11. Match the term to the appropriate 4. Breaks bonds between nitrogenous bases and separates DNA strands to initiate 5. A specalized RNA polymerase that creates a small fragment of replicated nucleotides (RNA primer) that provides a starting point for DNA replication 6. Recognizes the RNA primer and adds complimentary macleotides in the 5' to3 direction. 7. Removes RNA primer and nucleotides and replaces with DNA nucleotides 8. Forms a bond between two DNA nucleotides where gaps exist because of RNA primer replacement or Okazaki fragments. 9. The side of the replication fork with continuous synthesis 10. The side of the replieation fork with discontinuous synthesis. 11. Srnafragments of DNA nucleotides that are synthesized on the lagging strand 10 11 b. Lcading Strand c. DNA Helicase d. DNA PolymeraseI e. DNA Polymerase III f DNA Ligase g. Primase Email Save

Explanation / Answer

1) option b. Dna strand is always complimentary and antiparallel to each other. 5’-3’ and 3’-5’. Answer 2) option c purines are A and G , pyramidines are C and T(U) . Pairing occurs as AT and GC. Answer 3) This is because in semi-conservative DNA the newly synthesised helix consist of 1 daughter strand and one original parental strand. Answer 4) DNa helicase as it unwinds the dna and opens it for replication. Answer 5) primase is the Rna polymerase that adds primers to both thof strands. Answer 6) DNA pol lll adds nucleotides in 5’-3’ direction in both the strands. Answer 7) Dna pol I removes Rna primers having 3’-5’ exonuclease activity. Answer 8) Dna ligase helps in joining between the two nucleotides and seals the gap. Answer 9) continues synthesis always occus at leading strand which is 5’-3’ in direction. Answer 10) discontinpus synthesis always occurs at lagging strand which keep on adding primers. Answer 11) okazaki frangments present on lagging strand.