5’-GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCT-3’ 3’-CCCCAA-------------TCACCATCTCGCG

Question

5’-GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCT-3’ 3’-CCCCAA-------------TCACCATCTCGCGAACGGA-5’ a) In the above DNA sequence, what sequence will fit in the missing portion? b) If you allow one round of replication of the above DNA, how many times you will come across the following short DNA sequence? (5’-AGCT-3’) (5’-CCAT-3’) c) If the bold-typed region becomes a region for a primer, what will be the sequence of that primer?
d. What do you need for the replication of this DNA? e) Do you still need Ribonucleic acids for this replication? If so, then why? f) Count the number of A, G, C, T, and calculate the ratio of A to T and G to C.    5’-GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCT-3’ 3’-CCCCAA-------------TCACCATCTCGCGAACGGA-5’ a) In the above DNA sequence, what sequence will fit in the missing portion? b) If you allow one round of replication of the above DNA, how many times you will come across the following short DNA sequence? (5’-AGCT-3’) (5’-CCAT-3’) c) If the bold-typed region becomes a region for a primer, what will be the sequence of that primer?
d. What do you need for the replication of this DNA? e) Do you still need Ribonucleic acids for this replication? If so, then why? f) Count the number of A, G, C, T, and calculate the ratio of A to T and G to C.    5’-GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCT-3’ 3’-CCCCAA-------------TCACCATCTCGCGAACGGA-5’ a) In the above DNA sequence, what sequence will fit in the missing portion? b) If you allow one round of replication of the above DNA, how many times you will come across the following short DNA sequence? (5’-AGCT-3’) (5’-CCAT-3’) c) If the bold-typed region becomes a region for a primer, what will be the sequence of that primer?
d. What do you need for the replication of this DNA? e) Do you still need Ribonucleic acids for this replication? If so, then why? f) Count the number of A, G, C, T, and calculate the ratio of A to T and G to C. e) Do you still need Ribonucleic acids for this replication? If so, then why? f) Count the number of A, G, C, T, and calculate the ratio of A to T and G to C.   

Explanation / Answer

A- 5’-GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCT-3’

3’-CCCCAACCCCTAAATCGAGTCACCATCTCGCGAACGGA-5’

Missing DNA sequence is CCCCTAAATCGAG

B- In one round of DNA replication, we will encounter AGCT twice as this sequence is present in both the strand.

In one round of DNA replication, we will encounter CCAT once as this sequence is present only in bottom strand.

C- There is no bold typed sequence in the question.

D- we need to have the following material for DNA replication

DNA polymerase, Primers both reverse and forward. dNTPs, Buffer for DNA polymerase and the DNA.

E- SInce we are using the primers so we no more needed the Ribonucleic acid for the DNA replication. As we know that DNA polymerase cannot initiate the DNA replication its own. It needs a free -OH at 3' end which is provided by the primer that we have used. So we no longer need Ribonucleic acid for replication.

F- G - 16+6 = 22

C- 16+6 = 22

T 11+5 = 16

A -11+5 = 16

Total G - 22

C= 22

T - 16

T- 16

Ratio of A to T is 1

Ratio of G to C is 1

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