what type of cis acting sequence is most likely disrupted by these mutations in
ID: 213277 • Letter: W
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what type of cis acting sequence is most likely disrupted by these mutations in lactose intolerant people saplinglearning.com/ibiscms/mod/ibis/view.php?id 4835630 Learning Jump to... Gradeboo! 3/23/2018 11:59 PM Calculator dPenodic Table Question 9 of 12 Many adults become lactose intolerant later in life which is thought to be due to changes in transcription of the Map gené. With this hypothesis, you sequence the LCT gene and more of its surrounding genomic sequence in severa olerant and lactose intolerant individuals. You find a number of single basepair mutations only in the lactose intolerant individuals. These mutations are not in the LCT gene, but are located 10-20 kilobases upstream of the gene in the introns of the MCM6 gene (pictured below as bolded nucleotides with slash marks, example- GIA) MCM6 gene LCT gene cxons introns 3" CG/CTAAGT.... GATAAT/GGTAGC/TCCC/GTG. -14010bp 13915bp 13910bp 13907bp GATCCCT/CACTT 3712bp 2201abp here to search F4 F5Explanation / Answer
Answer: Option E is correct.
Explanation:
The changes in sequence are located outside the coding sequence of LCT gene. Hence the mutations must be located in cis-acting elements that are required for its proper expression.
5'-splice site is part of the coding sequence. Hence option A is incorrect.
5'-cap is added to the end of the coding sequence. Hence option C is incorrect.
CAP binding site may not be required for LCT expression in humans. Hence option D is incorrect.
Enhancers are generally located far upstream/downstream to the coding sequence. Hence option E is incorrect.
Transcription factors generally bind within 1-5 kb of the upstream sequence. Here, most of the mutations are beyond 5 kb. Hence option B is incorrect.
Enhancers are generally located far upstream/downstream to the coding sequence. Hence option E is correct.
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