Virtual Lab Blood Typing/Proceduré Blood Typing Activity go to the following web
ID: 213264 • Letter: V
Question
Virtual Lab Blood Typing/Proceduré Blood Typing Activity go to the following website and Data 2. complete the blood typing analysis for the three patients and record the data in the chart below: Reaction when Anti-A serum added Reaction when Anti-B serum Blood Type Patient Added Karyotyping activity 1. go to: www.biology.arizona.edu 2. click on human biology 3. click on web karyotypingtread the introductory information) 4. click on patient histories 5. complete the activity 6. print copies of the completed karyotypes (this can be done after you have completed all three karyotypes) 7. Do the post lab questions on the following page(not the one from the lab)Explanation / Answer
Blood group serology
blood group- is defined as the classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells .a series of related blood types constitutes a blood group system such as the Rh or ABO SYSTEM THE frequencies of the ABO and Rh types vary from population to population. there are 4 major blood groups determined by the presence or absence of two antigens (Aand B) on the THE SURFACE OF RED BLOOD CELLS. in addation to the A AND B ANTIGEN THERE IS A THIRD ANTIGEN CALLED THE Rh FACTOR WHICH CAN BE EITHER PRESENT MEANS POSITIVE OR ABSENT MEANS NEGATIVE
BLOOD GROUP ANTIGEN
A HAS ONLY A ANTIGEN ON RED CELL AND ANTIBODY B ON THE PLASMA
B HAS ONLY B ANTIGEN ON RED CELLS AND A ANTIBODY IN THE PLASMA
AB HAS BOTH A AND B ANTIGENS ON RED CELLS BUT NITHER AOR B ANTI BODY IN THE PLASMA
O HAS NITHER A NOR B ANTIGEN ON RED CELLS BUT BOTH A AND B ANTIBODY IN THE PLASMA
METHORD - mark at right slide of the glass slide as A and the left side as B followed by D put.
put one drop of antisera A ON THE SIDE a marked as a and one drop pof antisera b on the side mark b followed by anti D MARKED AS D.
ADD ONE DROP OF TEST BLOOD SAMPLE /20 % CELL SUSPENSION TO EACH ANTISERA..
MIXED THE BLOOD WITH THE REAGENT USING A CLEAN STICK. SPREAD THE MIXTURE OVER AN AREA OF 15 mm dimeter.
gently rotate the slide to and fro and llok for agglutation. . agglutation if present indicates a positive result.
ACCORDING TO QUESTION
PATIENT ANTI A ANTI B BLOOD GROUP
1 + VE -VE A
2 -VE +VE B
3 -VE -VE O
4 +VE +VE AB
+VE MEANS AGGLUTATION OR HEMOLYSIS. AND -VE MEANS NOT AGGLUTATION.
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