25. Current Tocal cells/an Fold Expansion Previous Total cells/om 12706 (am Doub
ID: 213257 • Letter: 2
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25. Current Tocal cells/an Fold Expansion Previous Total cells/om 12706 (am Doubling Time LnFold Expansion) | Doubling Time x Time remaining in culture 2 days (days) 0.59 26. What does It mean to be aseptic? 27. Microorganisms and pathogens such as bacteria, viruses, and archaea are the only concern in a clean room environment. TRUE / FALSE 28. isopropyl Alcohol (IPA) kills all microorganisms and cther potential contaminants. TRUE/ FALSE 29. Dead bacteria are still a potentially lethal contaminant. TRUE/ FALSE 30. How might you detect that a cell culture is contaminated? Dilutions 31. Why would you need to dilute something?Explanation / Answer
26..What is aseptic
The area which is sterilized with sterilizing agent and free from contamination caused by bacteria, virus and other harmful micro organism.
27.. false, in clean room other potent contaminant are also present, aerosol, yeast, fungi, chemical contaminant, humans cell contaminant (like skin cells, blood etc), foreign DNA sample.
28.. false. We need some other disinfectant in some cases where only alcohol is not enough to disinfect, as in case of HIV we need flame sterilization while working. Other contaminant like foreign DNA and some lipid layer of bacteria are not neutralized by only isopropanol.
29..true. bacteria can produce harmful toxics. So even if the bacteria are died, this toxic can be potent contaminant. Additionally, their dead proportions like surface protein and core DNA are still lethal.
30. physically we can detect contamination by observing a thin film of white bacterial or microorganism plaque in culture. We can also observe culture under microscope for detection of culture contaminant. As other contaminant can change the pH of media, we can observe the pH change which can give the idea of contamination.
31.. highly concentrated solutions or mircoorganism cultures are non reactive with some chemicals, because in highly saturated chemicals, the solute molecules are in high proportion, hence it will not give any surface area for other chemical to react. In microorganism culture we can not observe proper characteristics if the culture is highly conc. Hence serial dilution is required.
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