What would be a false positive in an ELISA assay that detected Ab to a viral pro

Question

What would be a false positive in an ELISA assay that detected Ab to a viral protein? Why might that happen (besides operator error)

What might be a false negative in an ELISA assay that detected the presence of viral proteins? Why might that happen (besides operator error)

Pregnant women are screened as Rubella, which is a mild RNA viral infection can cause serious birth defects and/or loss of the pregnancy. Do you think that the test measures presence of viral proteins in the individual or Ab to viral proteins or both? Explain your answer.

Why is it important to detect antibodies even in apparently healthy individuals?

How might a smear look if you had a parasitic infection for the last 6 months?

Explanation / Answer

False positive means in ELISA assay signifies that person is having the viral disease when infact the person does not have. False positive result may occur due to interferences caused by heterophilic antibodies.

False negative means in ELISA assay signifies that person is not having the viral disease when infact the person have that disease. False negative result may occur due to insufficient antigen coating of microtitre plates.

Test well be done for checking the antibodies for rubella virus. If mother had already in past, rubella disease than antibodies against the virus, will be there in the body but, if this not the case, mother should be vaccinated during pregnancy.

It is important because, antibodies signify that, your body is ready to fight against the germs and other infection in the body.

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