2. You have found a new streptomycin resistance allele in Streptococcus (the sam

Question

2. You have found a new streptomycin resistance allele in Streptococcus (the same bacteria that Avery and Griffiths were working with). A wild type version of this allele is normally found within the strain you are working in (hence it's normally streptomycin sensitive). When you clone this gene onto a plasmid in E. coli and then transform the original Streptococcus strain with this plasmid, you can isolate resistant Streptococcus colonies even though the plasmid does not replicate in Streptococcus When you PCR up this same gene and transform Streptococcus, you can NEVER select for resistant colonies. Describe one potential explanation for these experimental results. (12.5 pts)

Explanation / Answer

According to transforming principle of Griffith S strain (Smooth strain) and R strain (Rough Strain)

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