Record your results and whether they indicate a positive or negative reaction. R
ID: 212861 • Letter: R
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Record your results and whether they indicate a positive or negative reaction. Remember to record your color results within the first 2 minutes afterthe application of the oxidase reagent. Organism Result Producti of oxida Color of the bacterial sa mple Escherichia coli Pseudomonas aeruginosa Interpretation and Questions: 1. You were distracted by another student in the classroom and forgot about your oxidase test for 15 minutes. When you check your test result, you notice a dark blue ring but no added coloration to the colony smear. What can you conclude from this result 2. Which is more likely to cause infections of deeper, less oxygenated tissues: a member of the enterics or Pseudomonas? Why?Explanation / Answer
E.Coli is negative to oxidase reagent - Colour less or Light pink . Pseudomonas aeruginosa is positive to oxidase reagent - Dark purple or blue colour . 1. The blue ring developing in sample is not a positive but the colour due to reaction with paper. 2. Member of enterics can cause infection deeper in less oxygenated tissue , because they are faculative anaerobes they do not require oxygen. But pseudomonas require oxygen.
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