please explain Your crazy cousin Bob has designed a cloning project where he is

Question

please explain

Your crazy cousin Bob has designed a cloning project where he is inserting the C. elegans RICHES gene (named for a conserved amino acid domain) into a vector for expression in bacteria. Unfortunately, it's not working so well. You ask him to describe each of his steps. 4. Do a restriction digestion of the insert with the EcoRI enzyme a. b. Do a restriction digestion of the vector with the HindIII enzyme Hybridize the two pieces using the DNA ligase enzyme c. What advice would you give Bob? Be sure to explain to him what he's doing wrong. why that would not work, and what you would suggest instead.

Explanation / Answer

firstly i will suggest for following procedures:

A. Transformation

2. Plasmid Isolation

3.Restriction Digestion

4.Elution of the respective plasmid

5. Ligation of the insert and the plasmid.

and then check by transformation using the DH5alpha cells.

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