i. Consult our textbook for the genetic code. Examine the follewing nucleotide s

Question

i. Consult our textbook for the genetic code. Examine the follewing nucleotide sequence from the senae strand of DNA. What is the amino acid sequence of the encoded protein ATG-CCT-TAC-GCC-CCT-GGA-GAC-GAA-AAG-AAG-GGT disease. A type ofgene therapy called RNA interference (RNAD is being investigated to treat This disease is the result of a mutation in the DNA that results in the synthesis of a with an altered amino acid sequence. The mutated protein 2. forms dlumps, which causes nervous system treat this disease, scientists synthesize short sequences of RNA (sIRNA, or small Interfering RNA) that forms base pairs with the mRNA that codes for the mutated proteln Design an siRNA that will interfere with the synthesis of the protein shown in Model 2 b. Explain how the addition of the siRNA will prevent the synthesis of the mutated protein. c. What are the difficulties that must be overcome in order for RNA interference to be an effective technique for treating the disease oundations of Biochemisiry

Explanation / Answer

a) from sens strand to coding mRNA

UAC-GGA--AUG--CGG--GGA--CCU--CUG--CUU--UUC--CCA

Leu-- Gly--Met--Arg--Gly--Pro--Leu--Leu--Phe--Pro

2 a) The antisense strand is a perfect reverse complement of the intended target mRNA. if target mRNA is

UAC-GGA--AUG--CGG--GGA--CCU--CUG--CUU--UUC--CCA then its complimentary strand is act as a siRNA for this and bind with resulting the supression of target mRNA

2 C) from RNA interference gene technology ( like miRNA , si RNA etc) , we can suppress or degrade the coding mRNA which is reposible for the dieased proteins. RNA interference are targeted to its action and by supressing it we can slow down the diease or stop for further chronic condition of the dieases.

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