if you were to look at the proteins bound to the DNA at each region, in general

Question

if you were to look at the proteins bound to the DNA at each region, in general what types of proteins would you expect to see and why? What are the functions of these proteins in the cell?

5. The graph below shows the results of an experiment measuring levels of transcription for a specific yeast gene which has had regions of its promoter removed. The goal of this experiment is to determine which regions of the promoter contain enhancers, silencers, and the TATA box, all of which regulate transcription levels. +1 Region E (-800 to-1100) Region C (-150 to-400) Pegion B 100 to -150 Region A (-1 to -100) (-400 to -8001 Figure 1. Gene promoter region, showing 5 regions (A-El which are tested in the experiment 800 600 400 2 200 AD E Wild Type Yeast Strair Figure 2. Graph of transcription levels for yeast strains with regions A E deleted sequentially. (4 Indicates the region that has been remaved) torms hy mirht deleting part of the promoter region alter gene expression? (3 points)

Explanation / Answer

DNA binding proteins are the proteins which are mainly composed of various DNA- binding domains various motifs etc and thus have a specific or general affinity to either single or double-stranded DNA.

I expect DNA- binding proteins as they include various transcription factors which are able to modulate the process of transcription, various polymerases, nucleases which are able to cleave the DNA molecules, and histones which are involved various transcription in the cell nucleus and helps in the packaging of the chromosomes.

DNA-binding proteins can incorporate such domains as Zinc finger, the helix-helix turn and the leucine zipper these are the domains that facilitate binding to the nucleic acid.

DNA-binding proteins which help in :

1.non-specific DNA-protein interaction

2. DNA binding protein that specifically binds the single-stranded DNA

3.Helps in binding to specific DNA sequences

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