Subtitle NormalNo Spacing Heading 1 Heading 2 Title (1) Explain the role of each

Question

Subtitle NormalNo Spacing Heading 1 Heading 2 Title (1) Explain the role of each of the following in DNA replication: helicase (gyrase): DNA polymerase ligase: (2) Where does DNA replication start? (3) Where does it end? (4) DNA is antiparallel. What are the consequences of being antiparallel during replication? (5) DNA replication is saild to be semi-conservative. What does this mean? See it in action! (Protein synthesis in an eukaryote) http://www.youtube.com/watch?v- D3fOXt4MrOM (1) Where does transcription in prokaryotes occuri? (2) Where does transcription in eukaryotes occur? (3) A protein is encoded for by a stretch of nucleotides called a gene. In order for a gene to diret the synthesis of a protein, however, it must first be transcribed into RNA. What enzyme is responsible for synthesizing this RNA? 4) Where does transcription start? (5) Where does it end? Translation (1) Where does translation in prokaryotes occur? (2) Where does translation in eukaryotes occur? (3) What type of RNA is 'read' to synthesize a protein? (4) What "reads" the RNA to synthesize proteins? S) What specific site do the ribosomes recognize and bind to on the mRNA? (6) Using the provided mRNA sequence: a. Highlight and indicate where would the ribosome bind. b. Below the given mRNA sequence, indicate the sequence of the polypeptide that would be synthesized from the given mRNA. Make sure that you have 'established the right reading frame-that is, where would the ribosome start to translate this transcript? 5'-CGUAGGAGGUGGCUAUGUUGACAGUGUCGCCAGUUUCGUGAGUUACA -3 EO ,- Focus m + 100%

Explanation / Answer

1.
Helicase: unwinds the double stranded DNA so that DNA polymerase goes and synthesizes.
DNA polymerase: Enzyme which synthesizes new strand of DNA using a DNA template
Ligase: Joins different DNA strands together.

2.
DNA replication starts as soon as DNA polymerase finds AUG codon.
3.
DNA replication stops as soon as DNA polymerase finds stop codon.

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