Protein Name is : Concanavalin – A Uniport entery number is : P02866 i. For what

Question

Protein Name is : Concanavalin – A

Uniport entery number is : P02866

i. For what purpose is this protein produced by industry now ?

ii. In this modern day , how is this protein purified ? Present a detailed protocol for its purification as a flow chart

iii. Provide a detailed description of the molecular events and chemistry of each step in the procedure.

iv. Where possible, include and discuss column traces or stained gel images that indicate the purification achieved at specific steps.

v. References is required .

Explanation / Answer

i.

Concanavalin A is the lectin which, is extracted from the jack-bean. This is usually bind to the certain structures that are found in the different glycoproteins, glycolipids and sugars. Concanavalin A is a mitogen of plant. This has the ability to stimulate the T cell subsets of the mouse. Also, a suppressor T cell of the human is also sensitive to the Concanavalin A.

Concanavalin A has therapeutic effects against the hepatoma. This is the inhibitor of the hepatoma cells. Many pharmaceutical companies produce this protein for the purpose of treating hepatic cancer or producing it as antihepatic therapeutic agent.

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