6. (15 pts) Mitochondrial inner membranes can be agitated to generat like miniat

Question

6. (15 pts) Mitochondrial inner membranes can be agitated to generat like miniature spherical cells containing only a few membrane-bound proteins. Un mitochondria, the vesicles are oriented randomly, being either right-side-out or compared to the inner membrane (see below; the black structure is Fi three experiments, you make vesicles in the presence of adenine nucleotide soluti that the molecules get trapped inside the vesicles e vesicles, which are like inside-out -Fo ATP synthase ons, s ATP made 0n 1P Is on the DuBInside-out Right-Side-Out Vesicles Vesicles Experiment Nucleotide Solutions ADP + Pi ATP only ATP, ADP, Pi You take each vesicle preparation and acidify the solution with 0.01 M HCl. Experiment A vesicles are observed to synthesize 50% of the expected ATP (compared to mitochondria [i.e, right-side-out vesicles] with the same number of ATP synthases). (3 pts each) ) (3 pts) Why do the Experiment A vesicles only synthesize 50% of the expected ATP? All ATP is produced from inside-out vesicles, which are half of the total b All ATP is produced from right-side-out vesicles, which are half of the total 50% of the ATP synthases were lost during the agitation d. ATP produced by one type of vesicle is broken down by the other 3 pts) How would you treat Experiment B vesicles to hydrolyze ATP inside them? Increase the pH of the solution by adding base rasc base ecrease the pH of the solution by adding acid c. Add 2,4-DNP to remove the proton gradient d. ATP cannot be hydrolyzed in any Experiment B vesicles 3 pts) All statements about Experiment C vesicles are true, EXCEPT: a. [ATP] increases inside the right-side-out vesicles ATP] decreases inside the inside-out vesicles ATP] in the solution (outside of vesicles) decreases H+] increases inside both kinds of vesicles

Explanation / Answer

Ans 1-(b)All ATP is produced from right side out vesicle which are half of total.

And it is becauseRight side out vesicles are similar to the intact mitochondria That means the position of F0 and F1 is similar to mitochondria Which is required for proton gradient generation And atp production Hence the half number of right side out vesicles are going to produ e 50% of atp and the concentration of proton become low inside the cell..

Ans 2-(b)decrease the Ph of solutions by adding acid.

Because we know that Due to the acid-base properties of ATP, ADP, and inorganic phosphate, the hydrolysis of ATP has the effect of lowering the pH of the reaction medium. Under certain conditions,

Ans 3-(c)Atp in the solution (outside of vesicle) decrease.

And this is occur due to transfer of Atp into the cell by Which it gets energy by diffusion that's why the outer atp become decrease..

Hope u understand the answer.

Thank you

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