actose metabolic activity in the lac operon is represented in the image below bd

Question

actose metabolic activity in the lac operon is represented in the image below bd Determine the nature of the expression of the lacZYA constihutive, inducible, or no transcription in the presence of represented by L and label e each genotype as Assume that all promoters are wild type laci mutant cannot bind to the operator o laci mutant always brds to the operator Operator mutant thatl prevents repressor roL rocLF oLF the wild-bype operator o* and lact gene POL on a plasmid L lacZYA genes MacBook A 3 4 5

Explanation / Answer

I+O+Z+ - When lactose is present it will bind to the repressor as a result of it there will not be any hindrance on the operator site and Operon will be inducible.

I-O+Z+ -Since there is no repressor which binds to the operator region so the operon will be constitutively expressed.

I+OcZ+ F -Since In Oc repressor cannot bind to operon will be will be constitutively expressed.

I- O+Z+ F - operon in the cell cannot make the repressor protein but plasmid can make repressor protein which can bind to the operator region. But when lactose is present the operon can be induced. So operon is inducible.

IsO+Z+- Super repressor can bind to operator region even when lactose is present. So no transcription.

IsO+Z+ F- Super repressor can bind to operator region of the cell and the plasmid even when lactose is present. So no transcription.

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