22. The following enzyme catalyzed reaction occurs through a nucleophilic substi

Question

22. The following enzyme catalyzed reaction occurs through a nucleophilic substitution reaction (This is the reaction catalyzed by DNA polymerase). For this reaction, identify each of the following a. the nucleophile in the reaction b. the electrophile that is attacked by the nucleophile c. the 5' end of the product d. the 3'-end of the product e. a glycosidic bond 23. The diagram to the right represents the aminoacyl tRNA for a particular amino acid. CH, H-C-NH The figure below represents a fragment of mRNA that serves as a transcript for producing a protein. The sequence XYZ below binds to the tRNA molecule to the right. What is 1 possible sequence for XYZ? Be sure to consider the orientation (3'5, or 53') a. C-O 0 3'

Explanation / Answer

Que 22

The chain-elongation reaction catalyzed by DNA Polymerase.

a. The 3-hydroxyl group acts as a Nucleophile

b. Alpha phosphorus atom of the deoxynucleoside triphosphate act as a elctrophil

c. The 5' end of the free DNA nucleotide is added to the 3' end of the chain of nucleotides that is already synthesized.

5’ end product is Phosphate group

d. 3’ end product is OH group

e. Glycosidic Bond - In DNA, refers to the nitrogen-carbon linkage between the 9' nitrogen of purine bases or 1' nitrogen of pyrimidine bases and the 1' carbon of the sugar group.

Que. 23: The Possible sequence of XYZ is

3’CUU 5’ OR 3’UUU5’

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