1. H ow are the kinetics of an enzyme-catalyzed reaction affected by a purely no

Question

1. H ow are the kinetics of an enzyme-catalyzed reaction affected by a purely noncompetitive inhibitor? A) Vmax decreased, KM increased B) Vmax decreased, KM decreased C) Vmax decreased, KM unchanged D) Vmax unchanged, Ky increased E) Vmax unchanged, KM decreased 2. Which of the following is the primary building block for the synthesis of molecules like cholesterol or ubiquinone? A) acetyl-CoA B) palmitic acid C) choline D) isoprene E) sphingosine For a cell with Na*)out = 150 mM and [Na+],,-15 mN1 at 37 C, what is the free energy for movement of sodium ions from outside the cell to inside if the potential across the membrane is -60 mV? A) 11.7 kJ/mol B) 0.145 kJ/mol C) -0.145 kJ/mol D) -11.7 kJ/mol E) cannot be calculated from given information 3. 4. Which of the following terms describes all glycerophospholipid? A) zwitterionic B) amphipathic C) nonpolar D) amphoteric E) none of the above

Explanation / Answer

1) C- Vmax decreased, Km unchanged for an enzyme catalysed reaction affected by a purely noncompettitve inhibitor, the value of Vmax decreases as the inhibitor is only able to bind with tenzyme substrate complex, not the free enzyme,thus in the presence of inhibitor the value of Vmax decreases, as some enzymes will always be out for commission. In case of Km it is the measure of affinity of the enzyme for its substrate and thic could only be measured by an active enzyme, which means the fixed amountof inactive enzyme in noncompettive inhibiton does not affect Km.

2) acetyl Co-A is the primary building block for the synthesis of cholestrol or ubiquinone

4) B Glycerophospholipids are amphipathic i.e they have both the hydrophilic and hydrophobic parts.

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