Scientists have discovered a mosquito trapped in amber from 70 million years ago

Question

Scientists have discovered a mosquito trapped in amber from 70 million years ago, and that mosquito has fed on a dinosaur(!). In your attempt to garner a bonus point in Dr. H's genetics class, you are cloning a small piece of amplified dinosaur DNA. 11. What is the order of the following steps? (1-Grow transformed cell on a selective media.) (2-Add DNA ligase.) (3-Incubate chromosomal DNA and vector DNA with a restriction enzyme.) (4- Introduce the DNA into living cells.) (S-Mix the chromosomal DNA and vector DNA together) a. You go ahead and plate your bacteria on the selective media, but nothing grows. What do you think went wrong? b. You redo the experiment, and this time colonies grow on the plate, but they are all blue. What do you think went wrong? c.

Explanation / Answer

a. 5. Mix the vector DNA and chromosomal DNA together

3. Incubate chromosomal DNA and vector DNA with enzyme

2- Add DNA ligase

4. Introduce the DNA into living cell

1. Grow transformed cell on selective media.

b.If we can not see any colony growth on plate, there is a possibility that the ligation step did not work. DNA which are cut open by restriction enzyme are not ligated, they will not transform properly.

c.Blue colonies represents clones without the transformants, only the empty vector DNA. So if only blue colonies are grown, the possibility is that the transformation did not work properly.

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