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2. You have just received an urgent call from the Con ters for Disease Control a

ID: 209469 • Letter: 2

Question

2. You have just received an urgent call from the Con ters for Disease Control and Prevention (CDC) to con- ve on the following case of an unusual disease out- a sult on t Ibreak that they have been investigating. A new gram-positive bacterium related to Listeria monocyto- genes was isolated from an outbreak of food poisoning in Wisconsin due to contaminated cheese and appears to cause painful gastritis and in about half of exposed individuals, along with sudden onset of bleeding ul cers, death from toxic shock within 2 or 3 days. Upon biopsy of infected individuals, it was found that the bacteria were growing on the surfaces of epithelial cells lining the gastric pit of the stomach. Autopsy of individuals who died showed that bacteria were found only in the stomach and not in any of the other body organs. The researchers at the CDC have sub- sequently determined that, like L. monocytogenes, this new species of Listeria invades epithelial cells in tissue culture. Which, if any, of Koch's postulates have been satisfied for the involvement of this new Listeria strain in gastritis, bleeding ulcers, and toxic shock? additional measures could the research satisfy Koch's postulates? What ers take to help nerative, non

Explanation / Answer

The microorganism (species Listeria) are isolated from a diseased or dead animal.

Grown in a pure culture and the microorganisms in a colony is stained and identified under microscope.

The microorganisms grown in the pure culture are inoculated into a healthy laboratory animal.

The disease is reproduced in the animal.

The organism will be re-isolated from experimentally infected host and identified under microscope.

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