1 Detection of Ensyme Activity 1. Obeain a spot plate that has many small depres
ID: 209315 • Letter: 1
Question
1 Detection of Ensyme Activity 1. Obeain a spot plate that has many small depressions (wells) on its surface. 2· Place a drop ofíodine into each of the depressions. Each well will be used to detect the presence of starch. To the first well, add a drop of Deionized Water- this well serves as Obtain a test tube. Using the graduated pipette labeled "starch", fill it with 3 mL of the Using the graduated pipette labeled "amylase", add 1 ml of the amylase solution to the With a new pipette, immediately add 1 drop from the reaction tube to the second well your control. 3. starch solution. This is the reaction tube. 4. reaction tube 5. on the spot plate. A positive test for starch should be observed. Empty contents of the pipette back into the reaction tube. 6. Continue this procedure at 15 second intervals placing drops into consecutive wells. Continue this for a 2-minute period, and record the results in the Table following on the next page.Explanation / Answer
The control in this case is deionised water into which iodine is added. To this tube, amylase is not added. Amylase is added to the reaction tube. In this case, when iodine is added to the tubes of amylase, there would be a change in colour, to blue black. In order to confirm that the reaction has occurred and there is a change in colour, one tube is kept as control, without the enzyme.
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