2. You are a scientist working in a lab on a project to create starch from malto
ID: 209008 • Letter: 2
Question
2. You are a scientist working in a lab on a project to create starch from maltose. You have two tubes of identical, aqueous solutions, reactants, you regularly use in your work. You have labeled them with labelling tape and know that e and the other contains another solution. You were away from the lab for the only one of them contains your enzym weekend when the air conditioning failed in your lab and the labels have fallen off of your two identical aqueous tubes. You are unsure which is your enzyme of interest. Using the concepts of enzyme and the DNS methodology for visualization of the carbohydrate macromolecule, utilized today in lab, please write testable hypothesis to assign identity to the two tubes. Ho: HA: What experiment could you complete to test your hypothesis above? control group in your experimental design. Please provide an experimental group and Complete the following conversions: 1.0 mL 0.2 mL The absorbance of sample A is higher than the absorbance of sample B. Therefore, sample A has (circle the correct one) more less dissolved solute than sample B.Explanation / Answer
Answer:
Based on the given information, one of the tube contains enzyme and the second tube contains another solution. The DNS test (3,5-dinitrosalicylic acid) is used to detect the presence of reducing sugars such as glucose and fructose which contains aldehyde and ketone functional group respectively. Enzyme such as alpha amylase breaks down the starch molecule, thereby producing glucose which reacts with DNS and the formation of 3-amino-5-nitrosalicylic acid makes the solution appear pink.
Testable hypothesis to assign identity to two tubes.
Null Hypothesis: If the content of the tube are mixed with DNS solution and after incubation if the solution doesn't turn orange then the tube contains another solution.
Alternative Hypothesis: If the content of the tube are mixed with DNS solution and after incubation if the solution appears orange color then the tube contains the enzyme.
2) What experiment could you complete to test your hypothesis above.
Experiment: Which tube contains enzyme can be determined by performing DNS test using the content. DNS will react with reducing sugars and produce orange color.
Experimental group: Experimental group will consist of unknown tubes
Control group: Control group will consist of a known concentration of Enzyme
3) Complete the following conversions:
1.0mL = 1000 ul
0.2 mL = 200 uL
4) The absorbance of sample A is higher than the absorbance of sample B. Therefore, sample A has more dissolved solute than sample B
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