Please help me answer A and B in figure 2. Thanks! Preview File Edit View Go Too

Question

Please help me answer A and B in figure 2.
Thanks!

Preview File Edit View Go Tools Window Help 40% } Mon 4:48 AM Q E 401 PS2 022718-2.pdf (page 3 of 5)-Edited Q Search 401 PS2 022718-2.pdf 5· The DNA of certain viruses can be integrated into a cell's DNA as shown below in Figure 1 Figure 1 Integrated viral DNA You want to know the structure of the viral genome as it exists in the integrated state. You therefore perform a Southern Blot and obtain the data shown in Figure 2. To do the experiment, you use three different restriction endonucleases to digest viral DNA that was purified from virions (V in Figure 2, below) and DNA purified from cells that contain the integrated virus (C in Figure 2). After you separate the fragments by electrophoresis on an agarose gel, you blot the Biology 401, Spring 2018 Problem Set 2 DNA onto nitrocellulose and visualize the bands that contain viral DNA using a radioactively labelled viral DNA as a hybridization probe. After performing these operations, you obtain the patterns shown below: Bam H1 Eco R1 Figure 2 Sal 1 V C

Explanation / Answer

A.The viral genome will be incorporated into b segment

reason: We can see that the viral genome is integrated in the SalI site and the larger band is observed after integration, while two short bands are equivalent to the fraction created by cutting by ECORI and BamHI

B.In this case the RE will be available at the pit where the cross is made. It will open the circle and create a straight molecule to get incorporated as seen in the given diagram.

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.