You wish to study the protein product of a human gene, and decide to produce lar

Question

You wish to study the protein product of a human gene, and decide to produce large amounts of this protein by expressing the gene in E. coli.  As a source of donor DNAfor this project, the best choice would be:

Question 4 (1 point) You wish to study the protein product of a human gene, and decide to produce large amounts of this protein by expressing the gene in E. coli. As a source of donor DNA for this project, the best choice would be: a genomic clone of your human gene. a cDNA clone of your human gene. copies of the human gene of interest (derived from genomic DNA) that were amplified in vitro by PCR an expression plasmid with a human promoter. Any of the above.

Explanation / Answer

A cDNA clone will be the best choice because human genes are longer in length but E.coli can transcribed upto 40kd and also the pack size is limited. So if we take a cDNA clone then we sort out the Introns and only exons are present in the cloning part that saves the space and reduce burden for the E.coli cell

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