How would this mutation affect this enzyme? Hint: You will need to translate the

Question

How would this mutation affect this enzyme? Hint: You will need to translate the mRNA molecule beginning at its start codon. SHOW YOUR WORK.

QUESTION 10 (4.5 marks) A strain of E.coli requires adenine to grow because there is a mutation in one of the genes required for adenine synthesis The following shows part of the wild-type (normal) and mutant alleles of the gene. The bottom strand is the template for transcription. wild-type allele 5' .. .TTATGGGCAAGATCCCA .3' 3' .AATACCCGTICTAGGGT...5 mutant allele 5' .. . TTATGGGCTAGATCCCA.. .3' 3' ...AATACCCGATCTAGGGT .5

Explanation / Answer

Wild-type:
DNA sequence: 5'-TTATGGGCAAGATCCCA-3'
mRNA sequence: 5'-AAAUGGGCAAGAUCCCA-3'
Protein sequence: H2N-Met-Gly-Lys-Ile-Pro-COOH

Mutant:
DNA sequence: 5'-TTATGGGCTAGATCCCA-3'
mRNA sequence: 5'-AAAUGGGCUAGAUCCCA-3'
Protein sequence: H2N-Met-Gly-COOH

There is an A to T conversion in the mutant sequence (at the 9th position from the 5' end).
It is a transversion.
It is a nonsense mutation.
It creates a premature termination codon and produces a truncated peptide.

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