Based on the information and the graph given, What Is the NON-PROTEIN substance

Question

Based on the information and the graph given, What Is the NON-PROTEIN substance that is added? Explain Your Answer.

I am attempting to create the Glycolytic pathway (Glc->Pyruvate) in vitro. I purify all the enzymes of the pathway, the co-factors required, NAD+, ADP, ATP and P. I then mix all these components together and add Glucose. I then measure the ratio of Pyruvate:Glucose in the mixture over time. My data is shown in the graph. At the point marked "X" in the graph, I added a 2 substance to the pathway. Time

Explanation / Answer

Here non protein substance added is the GLUCOSE.

Initially you have lots glucose. Once glycolysis process started in vitro the pyruvate get synthesize. This lead to more pyruvate : glucose ratio. you will get exponential increase in the pyruvate : glucose ratio. After some time, glucose in the tube become limited then at that time pyruvate : glucose ratio will be same and have stationary stage.

when we add Substance X in the form of glucose, then once again more pyruvate get synthesize. you will again get exponential increase in the pyruvate : glucose ratio.

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