Lecture 12 Worksheet Name: eID: -Section: First Position s-end) Second Position

Question

Lecture 12 Worksheet Name: eID: -Section: First Position s-end) Second Position Third Position 3-end) UUU Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC SerUAC Tyr UCCCys C UGA Stop A UUA Leu UCA Ser UAA Stop UCC Ser UAG Sto CUU Leu CCU Pro CAU His CUC LeuCCC ProCAC His CAA Gln UUG Leu CCU Ang CGC Arg CGA Arg U CUA Leu CUG Leu CCA Pro AUC Ile AUA Ile AUG Met ACU Thr ACC Thr ACA Thr AAU Asn AAC Asn AAA Lys AGU SerU AGC Ser AGA Arg GCU Ala GCC Ala GAU Asp GAC Asp -CUCVial GGC Cly GUA ValGCA Ala GAA Glu GGA Gly GGG Gly GUG Val GCG Ala GAG Glu Using the genetic code provided, identify the amino acid sequence (using 1-or 3-letter codes) of the peptide encoded by the template DNA sequence shown below. 5'-TGCTACGAGATCGCTTGGTCGGACATTATCG-3

Explanation / Answer

Ans) the Amino Acid sequence coded by the given DNA sequence is AITGWFARAS

The information about the amino acid sequence in a protein is located in the DNA, is first copied into the mRNA by transcription processes, amino acid sequence information on mRNA present in the form of triplet codons.this triplet nature first proposed by the Crick. Ribosomes bind to the mRNA and translate this information (5’ to 3’ direction) as a protein by translation process. Each amino acid is coded by the three nucleotide bases of the mRNA. The presence of genetic code proved by the William Nierenberg and Har Gobind Khorana in 1961.

5’- TGCTACGAGATCGCTTGGTCGGACATTATCG-3’ (original template DNA strand in 5’ to 3’ direction)

3’-GCTATTACAGGCTGGTTCGCTAGAGCATCGT-5’ (original template DNA strand in 3’ to 5’ direction)

5’-GCUAUUACAGGCUGGUUCGCUAGAGCAUCGU-3’   (mRNA to be transcribed)

AITGWFARAS (Amino acid sequence)

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