VO4are etydrogenase d one strain is able to grow on glucose under both araerobic

Question

VO4are etydrogenase d one strain is able to grow on glucose under both araerobicard aerooc consors.nup .Voan omewhat slower than the wild type (normal) strain under aerobic condsitions Which srain Explain your reason 5 pts is this? ) A second mutant is unable to grow on glucose at all, regardless of whether or not Which strain is this? Again, explain your reasoning 4 pts lacking triose phosphate isomerase (which converts dihydroxyacetone phosphate to glyceraldehyde-3- phosphate in glycolysis) growing under aerobic conditions? 14. H ow many moles of ATP (or equivalent nucleotide) would be formed per mole of glucose in yeast oxidative phosphorylation. Enzyme names are not needed, but there should be a glear lustfication your answer Consider ATP generated at both substrate-level phosphorylation and via reducing equivalents during

Explanation / Answer

a) The strain which grows slower than the wild type strain under aerobic condition is pyruvate dehydrogenase mutant as because in the mutant strain,acetyl CoA is not formed from pyruvate,so TCA cycle can not occur,net ATP gain is reduced. But in the wild type strain ATP is produced more because of TCA cycle.

b) The second mutant which is unable to grow on glucose at all in both aerobic and anaerobic conditions,contains mutation in glyceralde-3-phosphate dehydrogenase gene. So glycolysis step is affected. 1,3-bisphosphoglycerate is not formed from glyceraldehyde-3-phosphate and further metabolism steps do not proceed.

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