Hello! 2. [9 points totall Another patient who has Usher syndrome was found to h

Question

Hello!

2. [9 points totall Another patient who has Usher syndrome was found to have no mutations in the harmonin gene. However, he was found to have a mutation in an miRNA called miRNA11, which gives the miRNA different binding properties than normal. Given what you know about the action of miRNAs A. (3 pts) Which lane on the gel below represents harmonin protein from the patient (the other lane shows protein from an unaffected individual)? Lane 2 1 2 100 50 35 25 20 15 10 kDa Harmonin protein -- B. (6 pts) Briefly explain your answer, describing how the miRNA works and what affect it has on mRNA and protein. the miRNA will bind to the mRNA for harmonin the harmonin mRNA will still be present, but the miRNA complexed with the harmonin mRNA will prevent translation, thus decreasing the amount of harmonin protein.

Explanation / Answer

Tho ugh there are no mutations in the harmonin gene , mutation in miRNA produces several harmonin isoforms some containing coiled domains . Because of several isoforms of harmonin, patient lane showed more protein.

B. Micro miRNA regulate post transcriptional modification by binding to 3' end of untranslated region of the complementary mRNA sequence.

Thus inturn repress the translation and bring about gene silencing

miRNA thus helps in RNA silencing and post trascriptional regulation of gene expression.

Changes in miRNA and its target site can deregulate gene expression.

It leads to various pathological conditions.

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